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New Validated Stablity Indicating Rp-Hplc Bioanalytical Method Development and Validation for Simultaneous Estimation of Hydrochlorothiazide, Ramipril and Losartan in Human Plasma by Using PDA Detector

Ashutosh Kumar S, Manidipa Debnath, Seshagiri Rao JVLN and Gowri Sankar D

A precised and reproducible stability indicating RP-HPLC method was developed for the simultaneous estimation of hydrochlorothiazide, ramipril and losartan potassium in plasma by using Symmetry C18 column (4.6 x 150mm, 5m, Make: Hypersil) in an isocratic mode. The drug was spiked in the plasma and extracted with mobile phase by protein precipitation method. The mobile phase was consisted of potassium dihydrogen phosphate (KH2PO4) and acetonitrile [HPLC Grade] in the ratio of 68:32 (% v/v). The detection was carried out at 210 nm. The percentage mean recoveries of hydrochlorothiazide, ramipril and losartan potassium were found to be 98.21-101.13, 98.82- 100.93 and 99.69-100.98 percentage respectively. This reveals that the method is quite accurate. The method was linear over the concentration range for hydrochlorothiazide 12.5-32.5, ramipril 1.25-3.25 and losartan 50.0 -130.0 μg/mL. The percentage relative standard deviation for inter-day and intra-day precision was found to be within limits. The lower limit of quantification was found to be 0.647, 1.283 and 2.647 μg/mL for hydrochlorothiazide, ramipril and losartan respectively. The percentage relative standard deviation obtained for the drugs spiked in plasma for stability studies were less than 2 %. The validation of method was carried out utilizing ICH-guidelines.