வாய்வழி சுகாதாரம் மற்றும் பல் மேலாண்மை

சுருக்கம்

Cariogenicity of Streptococcus mutans Glucan-Binding Protein Deletion Mutants

  David J Lynch, Suzanne M Michalek, Min Zhu, David Drake, Fang Qian and Jeffrey A Banas

Streptococcus mutans is a principal etiologic agent in the development of dental caries due to its exceptional aciduric and acidogenic properties and its ability to adhere and accumulate in large numbers on tooth surfaces in the presence of sucrose. Sucrose-dependent adherence is mediated by glucans, polymers of glucose synthesized from sucrose by glucosyl transferase (Gtf) enzymes. S. mutans makes several proteins that have the property of binding glucans. We hypothesized that three of these glucan-binding proteins (Gbps), Gbps A, C and D, contribute to the cariogenicity of S. mutans. A specific pathogen-free rat model was used to compare the cariogenicity of S. mutans UA130 and a panel of mutants with individual or multiple gbp gene deletions. The mutants were also evaluated in vitro for properties related to cariogenicity, such as acidogenicity, aciduricity, and adhesion to glucan. Only a subset of Gbp mutants were attenuated for cariogenicity, with the combined loss of Gbps A and C most affecting smooth surface caries. The attenuation of Gbp mutant strains was unlikely due to differences in acidrelated properties since the mutants were at least as acidogenic and acid-tolerant as the parental strain. Additionally, loss of Gbps did not reduce adhesion to a pre-formed biofilm of S. anguinis. Analyses of the caries data with in vitro biofilm properties previously determined for the mutant panel found correlations between cariogenicity and biofilm depth and substratum coverage. It is concluded that Gbps contribute to the cariogenicity of S. mutans through a mechanism that may involve alteration of biofilm architecture.