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Bioremediation of Trichlorpyr Butoxyethyl Ester (TBEE) in bioreactor using adapted Pseudomonas aeruginosa in scale up process technique

MH Fulekar*,M Geetha, J Sharma

Pseudomonas aeruginosa has been adapted at varying concentrations of pesticide - Trichlorpyr Butoxy Ethyl Ester (TBEE) viz. 10,25 50, 75 and 100 mg/l in MSM using incubator shaker at 37 ºC and 150 rpm by scale up process technique. In the beginning 10 mg/l concentration of TBEE was supplied to microorganism in minimal salt medium (MSM) under controlled conditions for 14 days. The culture was subsequently scaled up to higher concentration of TBEE by transferring one milliliter MSM containing 10 mg/l to 25 mg/l concentration in MSM and incubated for continuous 14 days. The Pseudomonas aeruginosa (NCIM 2074) was adapted in increasing concentration of TBEE at 50 mg/l, 75 mg/l and 100 mg/l; after every 14 days using a total period of 70 days. During adaptation period, the pseudomonas aeruginosa degraded the TBEE completely at concentrations of 10 mg/l and 25 mg/l; while 49% and 23% degradation in 75 mg/l and 100 mg/l of TBEE was observed. Hence bioremediation studies has been carried out at concentrations 10, 25 50 mg/l of TBEE using flask shake method. GCMS data showed that TBEE degraded 100% within 3 days at 10 mg/l concentration and 5 days at 25 mg/l initial concentrations. While in case of 50 mg/l initial concentration of TBEE the compound was found persisting till 8 days period. The major intermediated, during the bioremediation of TBEE, were found to be 3,5,6-trichloro-2-pyridinol and 2,4,6-trichloro benzene amine. These intermediates are less toxic than parent compounds which on long term acclimatization would convert into environment friendly compounds.