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Human Leukocyte HLA-B7 and HLA-B27 Antigen Typing among Healthy Adults Primedwith HIV-1 DNA Boosted with Recombinant Modified Vaccinia Ankara in Dar Es Salaam

Francis Millinga, Cyprian Mpinda

Background: The human leukocyte antigens are genetically inherited proteins present on thesurface of the cells of the immune system. Cells of the immune system recognize antigens only when presented to them next to an MHC self-molecule. Three main types of HLA Class I namely, HLA-A, HLA-B and HLA-C they are expressed by most human cells. Class I HLAs are involved in discriminating and presenting antigens to T-lymphocytes. T-lymphocytes kill the cell presenting it. T-lymphocytes destroy cells infected together with viruses, including HIV. Developing a vaccine for HIV may be aided by a complete understanding of those rare cases in which some HIV-infected individuals control replication of the virus. Most of these elite controllers express the histocompatibility alleles HLA-B57 or HLA-B27, these alleles remain the most robust associations with low concentrations of plasma virus.

Objectives: To evaluate the magnitude of Human leukocyte antigen production before and uponadministration of multiclade, multigene HIV-1 DNA prime/MVA boost among healthy individuals in Dar es Salaam, Tanzania.

Methods: EDTA plasma samples collected at baseline and post DNA prime and MVA boostimmunizations and archived in HIV vaccine immunogenicity study (HIVIS03) was used for HLA typing whereby HLA-B7 and HLA-B27 were determined using quantitative ELISA. Results were read and interpreted as per manufacturer’s instructions Data analysis was done using IBM SPSS software.

Results: A total of 42 samples from Police officers were tested at baseline and after HIV-1DNA MVA boost vaccine the mean age of tested subjects was 24 there were 34 males and 8 females,20 of them the vaccine was administered intradermally and 22 of them the vaccine was administered intramuscularly, educational level of participants was certificate of secondary education The mean, median and range of levels of HLA-B7 in males was Both HLA B7 andHLA B27 levels increased from baseline to vaccines group, but didn’t show significant difference between placebo and vaccines. Moreover HLA B7 and HLA B27 didn’t show association on mode of delivery of the vaccine, sex and age.

Conclusions: Both HLA-B7 and HLA-B27 were detected No statistically significant differencein levels we recommend further HLA typing using molecular or cellular methods.