மருத்துவ மற்றும் பரிசோதனை மருந்தியல் இதழ்

சுருக்கம்

Immunosuppressive Effects of Mesenchymal Stem Cells versus Corticosteroid in Experimental Model of Arthritis

El-denshary ESM ,LA Rashed ,M Elhussiny *

Objective: The objective of this study was to compare between the efficacies of mesenchymal stem cell (MSC)
and betamethasone in the treatment of rheumatoid arthritis.
Material and method: 90 male albino mice were divided equally into 2 models as follows: MSC model, group 1:
saline control group, group 2: Acetic acid, group 3: CIA, group 4: induced arthritis mice that received intravenous injection
of MSCs. Betamethasone model, group 1: phosphate buffer saline, group 2: Acetic acid, group 3: betamethasone
control, group 4: CIA, group 5: induced arthritis mice that received intraperitoneal injection of betamethasone. Mice
arthritis models were assessed by clinical paw edema and x-rays, at the proper time of sacrefaction, tissues were
collected and examined using real-time PCR (RT-PCR), synovial tissue was examined for (IL-10) interleukin-10
(TNF-α), tumor necrosis factor-α, (COMP)-Cartilage oligomeric matrix protein, (MMP-3) Matrix metalloproteinase3.
While (MDA), (LDH), (GSH), (CAT) and (MPO) were determined using colorimetric kits. In addition detection of serum
level of (IgG), (RF), (CRP), (ANA) by enzyme-linked immunosorbent assay (ELISA).Also detection of blood (ESR).
Results: Histopathological, paw edema and PCR results showed improvement of the group that received MSC
compared to the diseased group and group received betamethasone.
Conclusion: MSC significantly enhance the efficacy of collagen-induced arthritis treatment, which