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Isolation and Characterization of Siderophore and HCN Producing Pseudomonas aeruginosa from Indian Pompano Trachinotus mookalee Cuvier, 1832

Anu Mathew, Imelda Joseph, Susmitha V

Bacterial diversity study in marine fin-fish, Trachinotus mookalee Cuvier, 1832 lead to the isolation of six strains of Pseudomonas spp. The bacteria were isolated from the skin, gills and intestine of live fish collected from Karwar, Karnataka, India (N- 13°, 05.722‘; E- 079°, 48.658‘). Among the six strains three were non-fluorescent and three fluorescent Pseudomonas strains. Fluorescent strains were found to secrete pyoverdine, a yellow-green siderophore which has wide application in agriculture and allied sectors for use as biocontrol agent. Fluorescent strains were characterized phenotypically as well as by 16S rdna sequencing and were identified as Pseudomonas aeruginosa strain TRG1 (Genbank Accession No. KC109784).