மீன்வளர்ப்பு ஆராய்ச்சி மற்றும் மேம்பாட்டு இதழ்

சுருக்கம்

The Use of Indigenous Probiotic Halomonas aquamarina and Shewanella algae for White Shrimp (Litopenaeus vannamei Boone) Hatchery Productivity in Zero Water Discharge System

Gede Suantika *,Pingkan Aditiawati ,Dea Indriani Astuti ,Zarah Fazri Khotimah

This research was aimed to improve the performance of white shrimp postlarvae culture through the application of indigeneous probiotic bacteria, Halomonas aquamarina and Shewanella algae in zero water discharge system. The research was conducted by following two consecutive steps: (1) pathogenicity test of both probiotic in white shrimp culture, and (2) probiotic effect test to water quality and vibriosis syndrome control. From the first step, the use of probiotics had no pathogenocity effect to shrimp PL since survival rate of 84-98% was documented. From step two, the application of both probiotic bacteria was able to inhibit the population growth of V. harveyi in which the highest survival rate of 93.94% obtained from H. aquamarina addition, followed by S. algae addition (92.12%), H. aquamarina: S. algae addition (90.60%), S. algae: V. harveyi addition (89.39%), H. aquamarina: S. algae: V. harveyi addition (87.87%), H. aquamarina: V. harveyi addition (87.57%), no addition of bacteria (84.84%) and V. harveyi addition (82.42%). There was no significantly different (p>0.05) among the treatments on all water quality parameters which were still in tolerance range of white shrimp PL culture (salinity 26-30 ppt; temperature 26-28°C; pH 7.5-8.5; DO 5.7-6.4 mgL-1; ammonia 0.1-0.5 mgL-1; nitrite 0.02-0.25 mgL-1; nitrate 5-40 mgL-1). In term of other biological parameter, the use of these probiotics was significantly affecting the weight increase of shrimps. Bacterial identification showed that there was a major similarity of microbial diversity found both in water and L.vannamei intestine. It could be concluded that the use of H. aquamarina and S. algae as indigene probiotics contributed to the increase of shrimp survival rate. However this effect was not clearly described by the effect of water quality parameters improvement and it most probably due to the inhibition activity of these two probiotics on V. harveyi.